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#HEARTS OF IRON 4 TUTORIAL HOW TO#
It actually brings you In game and shows you how to do. These results also suggest that age-related conditions other than iron excess are responsible for the accumulation of hepatic macrophages with aging. Hoi4s tutorial isnt about little men telling you extremely vaguely. Double-staining experiments demonstrated that both M1 (iNOS +) and M2 (CD163 +) macrophages contained hemosiderin, suggesting that macrophages of both phenotypes stored iron. Aging resulted in significantly greater numbers of M1 (CD68 +) and M2 (CD163 +) macrophages in the liver, but neither macrophage number nor phenotype were affected by deferoxamine. Iron administration to young rats resulted in iron concentrations that matched the values and pattern of tissue iron deposition observed in aged animals however, iron did not alter macrophage number or phenotype.
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A separate group of old (24 mo) rats was treated with 200 mg/kg deferoxamine every 12 h for 4 days.
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To evaluate macrophages in a physiological model of iron loading that mimicked biological aging, young (6 mo) Fischer 344 rats were given one injection of iron dextran (15 mg/kg), and macrophage number and phenotype were evaluated via immunohistochemistry. Aging increases hepatic macrophage number and nonparenchymal iron, yet it is unknown whether age-related iron accumulation alters macrophage number or phenotype. Conversely, intracellular iron can alter macrophage phenotype. Liver macrophages serve important roles in iron homeostasis through phagocytosis of effete erythrocytes and the export of iron into the circulation.